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KMID : 0900919990230030205
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Korean journal of Animal Reproduction
1999 Volume.23 No. 3 p.205 ~ p.212
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Effects of Selenium and Vitamin E Administration on the Semen Characteristics, Blood Chemical Values and Hormone in Hanwoo Sires II. Changes of Selenium and Vitamin E Concentrations of Blood in Hanwoo Sires after Selenium, Vitamin E and rBST Administration
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Park Dong-Heon
Yang Boo-Keun
Kim Jong-Bok
Jeon G. J.
Cheong Hee-Tae
Park Choon-Keun
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Abstract
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The present study was designed to examine the effects of selenium(Se), vitamin E(Vit. E) and recombinant Bovine Somatotropin(rBST) administration on the selenium and vitamin E concentrations of blood in Hanwoo sires Hanwoo sires were randomly assigned to five groups; 1. control, 2. rBST, 0.09mg/kg body weight (BW) 3. Vit E, 1,500IU/kg BW, 4. Se 0.lmg/kg BW, 5. Vit E, 1.500IU plus Se 0.1mg/kg BW. rBST, Vit. E and Se for each experimental group were given 6 times at 15 days interval by intramuscular injection. Blood samples were collected 10 times for experimental periods, separated the serum by centrifugation, and stored at －70^{circ}C. Se and Vito E concentrations in blood were measured by fluorophotometer and HPLC. Se concentrations of blood in control, rBST, Vito E, Se and Se plus Vito E groups were 64.55, 65.50, 68.15, 73.11 and 74.09 ppb/mell, respectively. Se concentration in Vit. E plus Se group was significantly higher than in control and rBST groups (P＜0.05), but Vito E group was not significantly different in control and rBST groups(P＞0.05). The Vit. E concentrations of blood in control, rBST, Vit. E, Se and Se plus Vit. E groups were 2.27, 2.32, 2.80, 2.58 and 2.75 ppm/mell, respectively. Vit. E and Vit. E plus Se groups were slightly higher than those of any other groups, but not significantly difference in 외 I experimental groups(P＜0.05). These results indicate that Se and Vit. E concentrations of blood were slightly increased with the injection of Se and Vit. E in Hanwoo sires.
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KEYWORD
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Hanwoo sires, rBST, Vitamin E, Selenium, Blood
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